A quantitative, real-time polymerase chain reaction assay for beak and feather disease virus.

@article{Shearer2009AQR,
  title={A quantitative, real-time polymerase chain reaction assay for beak and feather disease virus.},
  author={Patrick L Shearer and Margaret Sharp and Nicolai J Bonne and Phillip Clark and Shane R Raidal},
  journal={Journal of virological methods},
  year={2009},
  volume={159 1},
  pages={98-104}
}
PCR-based assays for the detection of BFDV DNA are in widespread use throughout the world. Quantitative real-time PCR assays are extremely sensitive and have the advantages over standard PCR assays that they do not require post-reaction processing to visualise PCR products and can quantify the amount of DNA present in a sample. This study describes a quantitative real-time PCR assay for the detection of BFDV DNA, using primers designed to amplify a conserved 81 bp fragment of ORFV1 and SYTO9, a… CONTINUE READING