A putative monofunctional glycosyltransferase is expressed in Ralstonia eutropha.

@article{Paik1997APM,
  title={A putative monofunctional glycosyltransferase is expressed in Ralstonia eutropha.},
  author={Julie Paik and Dieter Jendrossek and Regine Hakenbeck},
  journal={Journal of bacteriology},
  year={1997},
  volume={179 12},
  pages={4061-5}
}
A gene, mgt, encoding a protein homologous to the N-terminal module of class A high-molecular-mass penicillin-binding proteins was identified in Ralstonia eutropha. By using specific antibodies, the corresponding Mgt protein was detected in association with the membrane, confirming that the N-terminal hydrophobic segment functioned as a membrane anchor. A derivative in which the hydrophobic sequence was deleted was overexpressed as a maltose-binding fusion protein in Escherichia coli. Cleavage… CONTINUE READING

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