A pulse-chase strategy for EdU labelling assay is able to rapidly quantify cell division orientation.

  title={A pulse-chase strategy for EdU labelling assay is able to rapidly quantify cell division orientation.},
  author={Xiaofeng Yin and H. Tsukaya},
  journal={The New phytologist},
  volume={211 4},
Measurement of the direction of cell division is an important, yet difficult, task to analyse how a plant organ acquires its final shape from an initially small group of cells. We introduce a method that rapidly and easily quantifies cell division direction and is applicable to all plant species. A pulse-chase strategy for 5-ethynyl-2'-deoxyuridine (EdU) labelling assay was established and was shown to be successful for leaves of Arabidopsis thaliana (Arabidopsis) and Juncus prismatocarpus. By… Expand
Importance of cell division angle, position of cell proliferative area, and localization of AN3 in lateral organ morphology
Results strongly indicate that lateral organ shapes are regulated via two aspects: position of meristem and cell division angles; the former is mainly governed by the AN3 expression pattern. Expand
ARGONAUTE10 is required for cell fate specification and the control of formative cell divisions in the Arabidopsis root meristem
It is shown that a functional miRNA gradient requires an opposing gradient of ARGONAUTE10, which sequesters miRNAs to protect HD-ZIP III transcripts from degradation, providing a framework for integration of phytohormone and miRNA-mediated patterning. Expand
Morphogenesis of flattened unifacial leaves in Juncus prismatocarpus (Juncaceae).
A diffuse 'thickening meristem' plays the key role in the development of flattened unifacial leaves as a result of the prolonged and diffuse distribution of thickening cell divisions. Expand
ANGUSTIFOLIA contributes to the regulation of three-dimensional morphogenesis in the liverwort Marchantia polymorpha
It is suggested that ANGUSTIFOLIA of Marchantia polymorpha acts as a coordinator of cortical microtubule orientations to regulate cell expansion in three-dimensional gametophyte morphogenesis, and contributes to three- dimensional morphogenesis by regulating cortical MT arrangement in the gamets of bryophytes. Expand
Has the impact of endoreduplication on cell size been overestimated?
  • H. Tsukaya
  • Biology, Medicine
  • The New phytologist
  • 2019
To date, several publications have referred to the relationship between the level of endoreduplication and cell size in Arabidopsis, claiming that enhanced/suppressed endocycling by mutation andExpand
Developmental analyses of divarications in leaves of an aquatic fern Microsorum pteropus and its varieties
This work utilized leaves of Microsorum pteropus, a semi-aquatic fern, and closely related varieties to analyze a variation in the divarication patterns, revealing localized growths and dissections of blades near each leaf apex. Expand
Leaf shape diversity with an emphasis on leaf contour variation, developmental background, and adaptation.
  • H. Tsukaya
  • Biology, Medicine
  • Seminars in cell & developmental biology
  • 2018
An overview of current knowledge is provided and future directions for the field of leaf contour are proposed, which can be considered marginal variation in curvature if the authors omit detailed marginal structures such as serrations. Expand
LY 6 K promotes cervical cancer growth , invasion and migration through regulating VEGFA
Purposes: To investigate the effect of proliferation, migration and invasion after knockdown LY6K in cervical cancer HeLa cells, and then clarify the potential mechanism. Methods: The expression ofExpand


A novel method to follow meiotic progression in Arabidopsis using confocal microscopy and 5-ethynyl-2′-deoxyuridine labeling
The increased number of meiotic staging criteria that can be assessed in confocal analysis, as compared to chromosome spreading or squashing, makes the identification of even the early and late portions of the prophase I substages attainable. Expand
A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine
EdU assay -which does not require plant cell wall digestion or DNA denaturation steps, offers reduced assay duration and better preservation of cellular, nuclear and chromosomal morphologies, and is shown to be an efficient tool for dual parameter flow cytometry analysis. Expand
A quantitative analysis of stem cell homeostasis in the Arabidopsis columella root cap
By labeling dividing stem cells with 5-ethynyl-2′-deoxyuridine (EdU), the number and distribution of their labeled progeny can be studied so that the division rate of stem cells can be measured quantitatively and the progression of stem cell progeny differentiation can be assessed in combination with Lugol’s staining. Expand
A maize root tip system to study DNA replication programmes in somatic and endocycling nuclei during plant development.
A promising new system that can be used to visualize DNA replication in isolated maize root tip nuclei after in planta pulse labelling with the thymidine analogue, 5-ethynyl-2'-deoxyuridine (EdU). Expand
The boundary of the meristematic and elongation zones in roots: endoreduplication precedes rapid cell expansion
Analyses of cells in the boundary region revealed that endoreduplication precedes rapid cell elongation in roots, and a boundary cell with 4C nuclei exhibiting the whole pattern of EdU signals was found. Expand
Cell division and cell expansion in cotyledons of Arabidopsis seedlings
An approach to use Arabidopsis cotyledons as an experimental system is demonstrated and a framework for molecular–genetic analysis of cellular dynamics during the growth of this organ is provided. Expand
Cell cycling and cell enlargement in developing leaves of Arabidopsis.
It is indicated that cell cycling related to leaf morphogenesis, tissue-specific patterns of cell proliferation, and cell differentiation occurs concurrently during leaf development and suggest that unique regulatory pathways may operate at each level. Expand
Key Proliferative Activity in the Junction between the Leaf Blade and Leaf Petiole of Arabidopsis1[W][OA]
Detailed analyses of the an3 and blade-on-petiole mutations further support the idea that organogenesis of the leaf blade and leaf petiole is critically dependent on the correct spatial regulation of the proliferative region of leaf primordia. Expand
Physcomitrella Cyclin-Dependent Kinase A Links Cell Cycle Reactivation to Other Cellular Changes during Reprogramming of Leaf Cells[C][W][OA]
A link between cell cycle reactivation and the acquisition of new cell-type characteristics through the activity of cyclin-dependent kinase A (CDKA) during reprogramming in the moss Physcomitrella patens is reported. Expand
Universal rule for the symmetric division of plant cells
This work shows that the selection of the plane of division involves a competition between alternative configurations whose geometries represent local area minima and posit that the new rule represents the default mechanism for plant cell division when internal or external cues are absent. Expand