A protonmotive force drives bacterial flagella.

  title={A protonmotive force drives bacterial flagella.},
  author={M. Manson and P. Tedesco and H. Berg and F. Harold and C. van der Drift},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={74 7},
Streptococcus strain V4051 is motile in the presence of glucose. The cells move steadily along smooth paths (run), jump about briefly with little net displacement (twiddle), and then run in new directions. They stop swimming when deprived of glucose. These cells become motile when an electrical potential or a pH gradient is imposed across the membrane. Starved cells suspended in a potassium-free medium respond to the addition of valinomycin by a brief period of vigorous twiddling. They also… Expand
The Response of Bacterial Flagellar Motor to Stepwise Increase in NaCl Concentration
The effect of NaCl on the output of the flagellar motor was significant and it may affect the cells in various ways, and how NaCl can be used to study various aspects of the bacterial flageLLar motor is explored. Expand
Flagellar motors of marine bacteria Halomonas are driven by both protons and sodium ions.
The results showed that the flagellar motors of the Halomonas strains were energized by both H+ and Na+ in one cell, which may reflect ecophysiological versatility to adapt to a wide range of salt conditions of the marine environment. Expand
The proton pump is a molecular engine of motile bacteria
Experiments are described demonstrating that bacterial motility can be supported by enzymatically generated or artificially imposed constituents of Δμ̄H+, that is, electric potential (ΔΨ) or Δ pH. Expand
Effect of Intracellular pH on Rotational Speed of Bacterial Flagellar Motors
It is proposed that the increase in the intracellular proton concentration interferes with the release of protons from the torque-generating units, resulting in slowing or stopping of the motors. Expand
Characterization of the Bacillus subtilis motile system driven by an artificially created proton motive force
Results suggest direct coupling of H+ influx to rotation of bacterial flagella through the induction of translational swimming in energy-depleted cells of Bacillus subtilis by an artificial proton motive force. Expand
Ion selectivity of the Vibrio alginolyticus flagellar motor
The marine bacterium, Vibrio alginolyticus, normally requires sodium for motility, but it is found that lithium will substitute for sodium and the coupling between ion transfers and force generation must be fairly tight. Expand
MotX, the channel component of the sodium-type flagellar motor
  • L. McCarter
  • Medicine, Biology
  • Journal of bacteriology
  • 1994
The deduced protein sequence for MotX shows no homology to its ion-conducting counterpart in the proton-driven motor; however, in possessing only one hydrophobic domain, it resembles other channels formed by small proteins with single membrane-spanning domains. Expand
Direction of flagellar rotation in bacterial cell envelopes
It is concluded that a cytoplasmic constituent is required for the expression of CW rotation (or repression of CCW rotation) in strains which are not defective in the switch but at one of the preceding functional steps of the chemotaxis machinery. Expand
A voltage clamp inhibits chemotaxis of Spirochaeta aurantia
Data indicate the the action of valinomycin as a voltage clamp serves to inhibit the chemotaxis of S. aurantia and provide evidence to support the suggestion that the mechanism of Chemotaxis in this organism involves the transduction of sensory signals in the form of membrane potential fluctuations. Expand
Isotope and thermal effects in chemiosmotic coupling to the flagellar motor of streptococcus
The torque generated by the flagellar motor of Streptococcus strain V4051 has been determined and it is concluded that the motor is a reversible engine driven by simple acid-base dissociation. Expand