A proton-coupled conformational switch of Escherichia coli 5S ribosomal RNA.

@article{Kao1980APC,
  title={A proton-coupled conformational switch of Escherichia coli 5S ribosomal RNA.},
  author={Th. Kao and D. M. Crothers},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1980},
  volume={77 6},
  pages={3360-4}
}
We report temperature-jump kinetic studies of the early melting transition of Escherichia coli 5S rRNA. A single measurable relaxation time tau, independent of concentration, was found at 266 nm. We monitored the transition temperature tm for this process (in the range from 0 to 40 degrees C) as a function of Mg2+, Na+, K+, spermidine, and H+ concentrations. Contrary to the usual effect of salts on nucleic acid stability, addition of mono- and multivalent counterions decreases tm for the early… CONTINUE READING

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