A protocol for polymerase chain reaction detection of Enterococcus faecalis and Enterococcus faecium from the root canal.

@article{Molander2002APF,
  title={A protocol for polymerase chain reaction detection of Enterococcus faecalis and Enterococcus faecium from the root canal.},
  author={Anders Molander and P G Lundquist and Panos N. Papapanou and Gunnar Dahl{\'e}n and Claes Reit},
  journal={International endodontic journal},
  year={2002},
  volume={35 1},
  pages={1-6}
}
AIM The present study was set up to develop a protocol for detection of Enterococcus faecalis and Enterococcus faecium from the root canal. METHODOLOGY A collection of type strains and clinical isolates ol E. faecalis and faecium was used. Specific polymerase chain reaction (PCR) primers targeted against the 16S/23S rDNA intergenic region were used and PCR reactions were set up. PCR products were run on TBE-agarose gel and analysed. The sensitivity of the PCR systems was studied using serial… CONTINUE READING

From This Paper

Topics from this paper.

Similar Papers

Loading similar papers…