A protocol for efficient transformation and regeneration of Carica papaya L.

@article{Cai1999APF,
  title={A protocol for efficient transformation and regeneration of Carica papaya L.},
  author={Wenqi Cai and Carol Gonsalves and Paula F. Tennant and Gustavo Ferm{\'i}n and Manoel T. Souza and Nonglak Sarindu and Fuh-Jyh Jan and H. Zhu and Dennis Gonsalves},
  journal={In Vitro Cellular & Developmental Biology - Plant},
  year={1999},
  volume={35},
  pages={61-69}
}
A reproducible and effective biolistic method for transforming papaya (Carica papaya L.) was developed with a transformation-regeneration system that targeted a thin layer of embryogenic tissue. The key factors in this protocol included: 1) spreading of young somatic embryo tissue that arose directly from excised immature zygotic embryos, followed by another spreading of the actively growing embryogenic tissue 3 d before biolistic transformation; 2) removal of kanamycin selection from all… CONTINUE READING