A protocol for Agrobacterium-mediated transformation of Brachypodium distachyon community standard line Bd21

@article{Alves2009APF,
  title={A protocol for Agrobacterium-mediated transformation of Brachypodium distachyon community standard line Bd21},
  author={Silvia Cristina Batezati Alves and Barbara Worland and Vera Thole and John W. Snape and Michael Webster Bevan and Philippe Vain},
  journal={Nature Protocols},
  year={2009},
  volume={4},
  pages={638-649}
}
Brachypodium distachyon is a novel model system for structural and functional genomics studies of temperate grasses because of its biological and genetic attributes. Recently, the genome sequence of the community standard line Bd21 has been released and the availability of an efficient transformation system is critical for the discovery and validation of the function of Brachypodium genes. Here, we provide an improved procedure for the facile and efficient Agrobacterium-mediated transformation… 
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References

SHOWING 1-10 OF 32 REFERENCES
Agrobacterium-mediated transformation of the temperate grass Brachypodium distachyon (genotype Bd21) for T-DNA insertional mutagenesis.
TLDR
A facile, efficient and rapid transformation system for Bd21 is described using Agrobacterium-mediated transformation of compact embryogenic calli derived from immature embryos to facilitate large-scale functional genomics research in this model system.
High-efficiency Agrobacterium-mediated transformation of Brachypodium distachyon inbred line Bd21-3
TLDR
The identification of a transformable inbred diploid line, Bd21-3, and the development of a transformation method with transformation efficiencies as high as 41% of co-cultivated calluses producing transgenic plants are described.
A high-throughput Agrobacterium-mediated transformation system for the grass model species Brachypodium distachyon L.
In the ongoing process of developing Brachypodium distachyon as a model plant for temperate cereals and forage grasses, we have developed a high-throughput Agrobacterium-mediated transformation
Agrobacterium-mediated transformation and inbred line development in the model grass Brachypodium distachyon
TLDR
The development of inbred lines from 27 accessions of Brachypodium are described and it is indicated that most of the lines contained an insertion at a single genetic locus.
A rapid and efficient transformation protocol for the grass Brachypodium distachyon
A fast and efficient microprojectile bombardment-mediated transformation protocol is reported for the grass species Brachypodium distachyon, a proposed alternative model plant to Oryza sativa for
Genetic Transformation of Wheat Mediated by Agrobacterium tumefaciens
TLDR
A rapid Agrobacterium tumefaciens-mediated transformation system for wheat was developed using freshly isolated immature embryos, precultured immature embryos and embryogenic calli as explants, and stable integration, expression, and inheritance of the transgenes were confirmed by molecular and genetic analysis.
Agrobacterium-mediated sorghum transformation
TLDR
The first report of successful use of Agrobacterium for production of stably transformed sorghum plants is reported, with the method yields a higher frequency of stable transformation that other methods reported previously.
Brachypodium distachyon. A new model system for functional genomics in grasses.
A new model for grass functional genomics is described based on Brachypodium distachyon, which in the evolution of the Pooideae diverged just prior to the clade of "core pooid" genera that contain
Plant regeneration and micropropagation of Brachypodium distachyon
TLDR
It was concluded that B. distachyon performed well in tissue culture and was suitable for further studies aimed at genetic transformation and its continued development as a model molecular genetic system.
Agrobacterium tumefaciens‐mediated barley transformation
Genetically transformed barley was produced by eco-cultivating immature embryo explants with Agrobacterium tumefaciens carrying a binary vector coding for chimaeric bacterial genes, bar and gus, and
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