A proteomics approach to understanding protein ubiquitination

  title={A proteomics approach to understanding protein ubiquitination},
  author={Junmin Peng and Daniel Schwartz and Joshua E. Elias and Carson C. Thoreen and Dongmei Cheng and Gerald T. Marsischky and Jeroen Roelofs and Daniel Finley and Steven P. Gygi},
  journal={Nature Biotechnology},
There is a growing need for techniques that can identify and characterize protein modifications on a large or global scale. We report here a proteomics approach to enrich, recover, and identify ubiquitin conjugates from Saccharomyces cerevisiae lysate. Ubiquitin conjugates from a strain expressing 6xHis-tagged ubiquitin were isolated, proteolyzed with trypsin and analyzed by multidimensional liquid chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) for amino acid sequence… 

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