A proteomic approach to the identification of cytochrome P450 isoforms in male and female rat liver by nanoscale liquid chromatography-electrospray ionization-tandem mass spectrometry.

@article{Nisar2004APA,
  title={A proteomic approach to the identification of cytochrome P450 isoforms in male and female rat liver by nanoscale liquid chromatography-electrospray ionization-tandem mass spectrometry.},
  author={Saira Nisar and Catherine S. Lane and Andrew F Wilderspin and Kevin J. Welham and William J Griffiths and Laurence H. Patterson},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={2004},
  volume={32 4},
  pages={382-6}
}
Nanoscale reversed-phase liquid chromatography (LC) combined with electrospray ionization-tandem mass spectrometry (ESI-MS/MS) has been used as a method for the direct identification of multiple cytochrome P450 (P450) isoforms found in male and female rat liver. In this targeted proteomic approach, rat liver microsomes were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by in-gel tryptic digestion of the proteins present in the 48- to 62-kDa bands. The resultant… CONTINUE READING
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