A proteomic approach to study Cry1Ac binding proteins and their alterations in resistant Heliothis virescens larvae.

@article{JuratFuentes2007APA,
  title={A proteomic approach to study Cry1Ac binding proteins and their alterations in resistant Heliothis virescens larvae.},
  author={Juan Luis Jurat-Fuentes and Michael J Adang},
  journal={Journal of invertebrate pathology},
  year={2007},
  volume={95 3},
  pages={187-91}
}
Binding of the Bacillus thuringiensis Cry1Ac toxin to specific receptors in the midgut brush border membrane is required for toxicity. Alteration of these receptors is the most reported mechanism of resistance. We used a proteomic approach to identify Cry1Ac binding proteins from intestinal brush border membrane (BBM) prepared from Heliothis virescens larvae. Cry1Ac binding BBM proteins were detected in 2D blots and identified using peptide mass fingerprinting (PMF) or de novo sequencing. Among… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-9 of 9 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 25 references

Global status of commercialized biotech/GM crops

C. James
ISAAA briefs No. 34 • 2005
View 1 Excerpt

Characterization of cultured insect cells selected by Bacillus thuringiensis crystal toxin

In Vitro Cellular & Developmental Biology - Animal • 2004
View 1 Excerpt

Similar Papers

Loading similar papers…