A protein which binds preferentially to single-stranded core sequence of autonomously replicating sequence is essential for respiratory function in mitochondrial of Saccharomyces cerevisiae.

@article{Yamazoe1994APW,
  title={A protein which binds preferentially to single-stranded core sequence of autonomously replicating sequence is essential for respiratory function in mitochondrial of Saccharomyces cerevisiae.},
  author={Mitsuyoshi Yamazoe and Katsuhiko Shirahige and Mooza Rashid and Yoshinobu Kaneko and Takeshi Nakayama and Naotake Ogasawara and Hirofumi Yoshikawa},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 21},
  pages={15244-52}
}
From yeast nuclear extract, we have identified several DNA-protein complexes using the T-rich strand of core consensus sequence of autonomously replicating sequence by gel shift assay. One of them showed preferential binding to the T-rich sequence of the DNA. We have partially purified a protein constituent of this complex and cloned its gene. The gene has an open reading frame encoding a protein of 380 amino acids (M(r) = 42,100) which is processed to a mature protein of 371 amino acids (M(r… CONTINUE READING
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