A protein interaction map of soybean mosaic virus strain G7H based on the yeast two-hybrid system.

@article{Kang2004API,
  title={A protein interaction map of soybean mosaic virus strain G7H based on the yeast two-hybrid system.},
  author={Sung-Hwan Kang and W. Lim and K. -Y. Kim},
  journal={Molecules and cells},
  year={2004},
  volume={18 1},
  pages={122-6}
}
A protein interaction map of Soybean mosaic virus (SMV) strain G7H was generated by the yeast two-hybrid system. Clones encoding the genes P1, HC-Pro, P3, 6K1, CI, 6K2, VPg, NIa, NIb, and CP were fused downstream of the GAL4 binding domain (GAL4-BD) and of the GAL4 activation domain (GAL4-AD). The GAL4-BD and GAL4-AD fusion derivatives of each gene were co-transformed into yeast and transformants in which interaction took place were identified on selective media. Interacting fusion proteins… CONTINUE READING

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