A protein binding assay for adenosine 3':5'-cyclic monophosphate.

@article{Gilman1970APB,
  title={A protein binding assay for adenosine 3':5'-cyclic monophosphate.},
  author={Alfred G. Gilman},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1970},
  volume={67 1},
  pages={
          305-12
        }
}
  • A. Gilman
  • Published 1 September 1970
  • Biology, Chemistry
  • Proceedings of the National Academy of Sciences of the United States of America
A simple and sensitive assay for adenosine 3':5'-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10(-9) M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP. 

Figures and Tables from this paper

A Simple Competitive Protein-Binding Assay for Cyclic Adenosine 3´:5´-monophosphate

A cyclic adenosine 3': 5'-monophosphate (Ado-3':5'-P) assay is proposed basing on the principle of competitive protein-binding and a new method for linearisation of the dose response curve has the advantages of simplicity and ease of curve calculation.

A protein-binding assay for direct determination of adenosine 3',5'-monophosphate in amniotic fluid, cerebrospinal fluid, plasma, and urine.

  • L. AksnesO. Sövik
  • Biology, Chemistry
    Scandinavian journal of clinical and laboratory investigation
  • 1976
An adenosine 3',5'-monophosphate (cyclic AMP)-binding protein was isolated from bovine skeletal muscle and gave a sensitive and specific competitive protein-binding assay that permitted direct determination of cyclicAMP in the biological fluids mentioned above.

Adenosine 3' :5' monophosphate receptor sites in Xenopus laevis oocytes.

Adenosine 3':5' cyclic monophosphate (cyclic AMP) binds to proteins present in the supernatant fractions of full-grown oocytes of Xenopus laevis and remains constant during meiotic maturation induced by progesterone.

A simplified procedure for the assay of adenosine 3':5'-monophosphate by the activation of liver phosphorylase.

The method devised by Butcher et al. (1965) for the determination of adenosine 3′:5′-monophosphate (cyclic AMP) and based on the ability of the nucleotide to enhance the rate of activation of liver

A rapid and simple procedure for the determination of guanosine 3′,5′-monophosphate by use of the protein-binding method

  • V. Dinnendahl
  • Biology
    Naunyn-Schmiedeberg's Archives of Pharmacology
  • 2004
SummaryA modification of the protein-binding assay for guanosine 3′,5′-monophosphate (Murad et al., 1971) is decribed yielding a substantial increase in sensitivity together with a more simplified

Inhibition of adenosine 3',5'-monophosphate-dependent protein kinase by staphylococcal alpha-toxin

Staphylococcal alpha-toxin inhibited the activity of cyclic adenosine 3',5'-monophosphate-dependent protein kinase by competitive inhibition, probably by its interaction with a cyclic adsorption site in the protein Kinase molecule.

Measurements of rates of adenosine 3':5'-cyclic monophosphate synthesis in intact Escherichia coli B.

  • A. PeterkofskyC. Gazdar
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1973
Under all conditions tested, the adenylate cyclase activity in vivo is great enough to account for the pattern of accumulation of cyclic AMP, in contrast to previous adenosine 3':5'-cyclic monophosphate assays in vitro, where the measured enzyme activities were insufficient to accounts for the amounts of cycled AMP.

Fluorescent Modification of Adenosine 3',5'-Monophosphate: Spectroscopic Properties and Activity in Enzyme Systems

1,N6-ethenoadenosine 3',5'-monophosphate is indicated by its long fluorescent lifetime, detectability at low concentration, and relatively long wavelength of excitation (300 nanometers).

Use of affinity elution chromatography for the microassay of adenosine 3':5'-monophosphate.

The degree of interference of different nucleotides including cyclic GMP was found to be minimal and in this respect the use of affinity elution chromatography was comparable to radioimmunoassay.

Adenosine 3',5'-monophosphate (cAMP)-binding protein and cAMP-dependent protein kinase in human placenta.

cAMP modulates estrogen, hCG, and lactate syntheses by human placenta, cAMP presumably exerts its major intracellular effect by binding to cAMP-dependent protein kinase (cAMP-PK), which, in turn,
...