A protein binding assay for adenosine 3':5'-cyclic monophosphate.

  title={A protein binding assay for adenosine 3':5'-cyclic monophosphate.},
  author={Alfred G. Gilman},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={67 1},
  • A. Gilman
  • Published 1 September 1970
  • Biology, Chemistry
  • Proceedings of the National Academy of Sciences of the United States of America
A simple and sensitive assay for adenosine 3':5'-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10(-9) M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP. 

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