A proportional analysis method using non-kinetic real-time PCR.

@article{Suyama2007APA,
  title={A proportional analysis method using non-kinetic real-time PCR.},
  author={Tetsushi Suyama and Nana Matsuura and Takahiro Kanagawa},
  journal={Journal of biotechnology},
  year={2007},
  volume={128 1},
  pages={
          41-9
        }
}
Prompted by increasing interest in proportional analysis of genetic types, we developed a simple assay technique for determining the ratio of a specific target gene in the total genes that can be amplified with the same PCR primer. The key feature of this method is that the following two tasks are performed in a single-tube real-time PCR system: task 1, PCR amplification of the total genes including the target using a labeled PCR primer, with concurrent monitoring of the total copy number of… CONTINUE READING

From This Paper

Topics from this paper.