A prolactin-inhibiting factor within the precursor for human gonadotropin-releasing hormone

@article{Nikolics1985APF,
  title={A prolactin-inhibiting factor within the precursor for human gonadotropin-releasing hormone},
  author={K{\'a}roly Nikolics and Anthony John Mason and {\'E}va Szőnyi and Janakiraman Ramachandran and P. H. Seeburg},
  journal={Nature},
  year={1985},
  volume={316},
  pages={511-517}
}
The cloned complementary DNA sequence encoding the human gonadotropin-releasing hormone (GnRH) precursor protein was used to construct an expression vector for the bacterial synthesis of the 56-amino acid GnRH-associated peptide (GAP). GAP was found to be a potent inhibitor of prolactin secretion and to stimulate the release of gonadotropins in rat pituitary cell cultures. Active immunization with peptides corresponding to GAP sequences led to greatly increased prolactin secretion in rabbits. 
The Biosynthetic Precursor of Gonadotropin-Releasing Hormone: A Multifunctional Prohormone
TLDR
Mammalian reproduction is regulated by the pituitary hormones luteinizing hormone (LH), follicle stimulating hormone (FSH), and prolactin, which stimulates ovulation and corpus luteum formation in females and androgen secretion in males.
Evolution of gonadotropin-releasing hormones
Gonadotropin-Releasing Hormone-associated Peptide Immunoreactivity in Bovine Colostrum
TLDR
The presence of GAP immunoreactivity in bovine colostrum suggests that the GnRH precursor is synthesized and processed in mammary tissue itself, and is of low molecular weight and a high hydrophobic character.
The gonadotropin-releasing hormone associated peptide reduces calcium entry in prolactin-secreting cells.
TLDR
It is shown that GAP reduces intracellular Ca++ levels, [Ca++]i, and inhibits Ca++ transients in these cells and may explain the inhibitory effect of GAP on hormonal secretion by PRL-secreting cells.
Partial purification of a hypothalamic factor that inhibits gonadotropin-releasing hormone-stimulated luteinizing hormone release.
A protein that suppresses GnRH-stimulated LH release from dispersed rat anterior pituitary cells was purified from rat hypothalami by chromatography on Sephadex G-25, carboxymethyl cellulose, and
Biological Activity of Non-GnRH Synthetic Peptide Sequences of the GnRH Precursor
The demonstration of GnRH biosynthesis by tryptic and carboxypeptidase B cleavage of a higher molecular precursor in sheep, pig and rat hypothalamus, and the postulate that pGlu1 is derived from
The GnRH family of peptides
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