A prokaryotic-type cytidine deaminase from Arabidopsis thaliana gene expression and functional characterization.

@article{FaivreNitschke1999APC,
  title={A prokaryotic-type cytidine deaminase from Arabidopsis thaliana gene expression and functional characterization.},
  author={S E Faivre-Nitschke and J. M. Grienenberger and Jos{\'e} M. Gualberto},
  journal={European journal of biochemistry},
  year={1999},
  volume={263 3},
  pages={896-903}
}
The gene and cDNA of an Arabidopsis thaliana cytidine deaminase (CDA) were cloned and sequenced. The gene, At-cda1, is located on chromosome 2 and is expressed in all plant tissues tested, although with quantitative differences. Expression analysis suggest that At-cda1 probably codes for the housekeeping cytidine deaminase of Arabidopsis. The gene was functionally expressed in Escherichia coli and the protein, At-CDA1, shows similar enzymatic and substrate specificities as conventional cytidine… CONTINUE READING

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