A proinsulin 74-90-derived protease-resistant, altered peptide ligand increases TGF-beta 1 secretion in PBMC from patients with type 1 diabetes mellitus.

@article{Aalst2010AP7,
  title={A proinsulin 74-90-derived protease-resistant, altered peptide ligand increases TGF-beta 1 secretion in PBMC from patients with type 1 diabetes mellitus.},
  author={Denise van Aalst and Hubert Kalbacher and David Palesch and Fang Zou and Andreas Spyrantis and Silke Rosinger and B O Boehm and Timo Burster},
  journal={Journal of leukocyte biology},
  year={2010},
  volume={87 5},
  pages={943-8}
}
Proinsulin is a major diabetes-associated autoantigen. APL have been shown to manipulate the immune response of T cells. Here, we generated a lysosomal protease-resistant proinsulin 74-90-derived APL using a CS-directed amino acid modification approach. These prAPL activated TGF-beta 1 secretion in proinsulin-reactive T cells from PBMC of patients with T1D. We provide evidence that proinsulin-derived prAPL modulate the cytokine signature of proinsulin-reactive T cells at a micromolar range by… CONTINUE READING