A production of monoclonal antibodies by a simple electrofusion technique induced by ac pulses.

@article{Takahashi1991APO,
  title={A production of monoclonal antibodies by a simple electrofusion technique induced by ac pulses.},
  author={Yasuo Takahashi and Koji A. O. Suzuki and Takahide Niimura and Takeshi Kano and Sachiko Takashima},
  journal={Biotechnology and bioengineering},
  year={1991},
  volume={37 8},
  pages={790-4}
}
A simple and effective technique of electrofusion of the mouse myeloma cells and lymphocytes induced by AC pulses was investigated. Instead of pearl chain formation, we used a mild centrifugation of high-density cell suspensions to enhance the cell-cell contacts. The optimal frequency of the AC field used for cell fusion was 10 kHz. We confirmed the production of monoclonal antibodies against human serum albumin (HSA) by the hybridoma obtained by this method. Under the conditions used in the… CONTINUE READING

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