A procedure for the rapid freezing of whole embryos

@article{Newgreen1977APF,
  title={A procedure for the rapid freezing of whole embryos},
  author={Donald F. Newgreen and I. J. Allan},
  journal={Experientia},
  year={1977},
  volume={33},
  pages={1513-1514}
}
A procedure for the rapid freezing of whole chick embryos for histochemical treatment is described. The problems of deformation during preparation for quenching and orientation for sectioning have been largely overcome by placing embryos inside lengths of chicken trachea. The subsequenct disorientation of tissues that follows cracking and shattering due to… CONTINUE READING