A procedure for selecting and culturing subpopulations of neurons from rat dorsal root ganglia using magnetic beads.

@article{Tucker2005APF,
  title={A procedure for selecting and culturing subpopulations of neurons from rat dorsal root ganglia using magnetic beads.},
  author={Budd A Tucker and Masuma Rahimtula and Karen M. Mearow},
  journal={Brain research. Brain research protocols},
  year={2005},
  volume={16 1-3},
  pages={50-7}
}
Current protocols for preparing primary sensory neuron cultures are inadequate when studying individual subpopulations of dorsal root ganglion (DRG) neurons. The DRG is made up of a heterogeneous population of cells, making it difficult to study treatment effects on any given population in mass cultures. Thus, we describe a procedure using magnetic beads from Dynal to select and plate viable populations of neurons based on expression of specific cell surface markers. We show that, by the use of… CONTINUE READING
7 Citations
0 References
Similar Papers

Similar Papers

Loading similar papers…