A probability-based approach for high-throughput protein phosphorylation analysis and site localization

@article{Beausoleil2006APA,
  title={A probability-based approach for high-throughput protein phosphorylation analysis and site localization},
  author={Sean A. Beausoleil and Judit Vill{\'e}n and Scott A. Gerber and John Rush and Steven P. Gygi},
  journal={Nature Biotechnology},
  year={2006},
  volume={24},
  pages={1285-1292}
}
Data analysis and interpretation remain major logistical challenges when attempting to identify large numbers of protein phosphorylation sites by nanoscale reverse-phase liquid chromatography/tandem mass spectrometry (LC-MS/MS) (Supplementary Figure 1 online). In this report we address challenges that are often only addressable by laborious manual validation, including data set error, data set sensitivity and phosphorylation site localization. We provide a large-scale phosphorylation data set… 

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