A primer pair for amplifying part of the genome of all potyvirids by RT-PCR.

@article{Gibbs1997APP,
  title={A primer pair for amplifying part of the genome of all potyvirids by RT-PCR.},
  author={Adrian J. Gibbs and Anne M. Mackenzie},
  journal={Journal of virological methods},
  year={1997},
  volume={63 1-2},
  pages={9-16}
}
Sequence analysis was used to design a pair of degenerate oligonucleotide primers that amplified a 1.6-2.1 kbp fragment from the 3' end of the genome (virion protein gene and part of the NIb gene) of 17 species of the Potyviridae ('potyvirids'); 11 potyviruses, 2 bymoviruses, 2 macluraviruses, an ipomovirus and a rymovirus. The 'potyvirid primer 1' hybridizes to the 3' terminal poly-A region of the genome, and 'potyvirid primer 2' to the genomic region encoding the-GNNSGQ-motif of the NIb… CONTINUE READING
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