PURPOSE To evaluate the effectiveness of atelocollagen for canine corneal wound healing. MATERIALS AND METHODS Atelocollagen was used to fill a transplant bed in the central cornea, which was then covered with a contact lens. The wound healing process was analyzed clinically, morphologically, and biochemically. RESULTS At the early healing stage, both the pupillary zone and details of the iris were observed. The stromal collagen fibrils normalized in a time-dependent manner. Type III collagen in the wound area was detected faintly throughout the experimental period. CONCLUSIONS This novel method is advantageous for accelerating wound healing without causing inflammation.