BACKGROUND Darvyadi Yoni Varti is an Ayurvedic formulation indicated for the management of Garbhasaya Grivamukhagata Vrana (cervical erosion). Though a number of drugs are available in modern medicine, interest towards Ayurveda is gaining. Drugs with Vranasodhana, Ropana and Prasadana properties like Sphatika, Darvi, Haridra, and Lodhra are useful in this situation and are selected to prepare Yoni Varti. AIM To standardize and evaluate preliminary pharmacognostical as well as physicochemical profiles of Darvyadi Yoni Varti. MATERIALS AND METHODS Raw material was analyzed in the Pharmacognostical Laboratory for genuinity before converting into Varti. Finished product was analyzed by following pharmacopoeial standards. RESULTS The pH value of Darvyadi Yoni Varti was 5.0, water soluble extract was 23.3%w/w, alcohol soluble extract was 8.7%w/w, ash value was 5.16%w/w, loss on drying was 71.73%w/w. High performance thin layer chromatography was carried out; in which maximum 10 spots at 254 nm and 3 spots at 366 nm were distinguished. CONCLUSION Pharmacognostical study of raw drug revealed the quality and genuineness of all the constituents of Darvyadi Yoni Varti.