A precise spectrophotometric method for measuring sodium dodecyl sulfate concentration.

Abstract

Sodium dodecyl sulfate (SDS) is used to denature and solubilize proteins, especially membrane and other hydrophobic proteins. A quantitative method to determine the concentration of SDS using the dye Stains-All is known. However, this method lacks the accuracy and reproducibility necessary for use with protein solutions where SDS concentration is a critical… (More)
DOI: 10.1016/j.ab.2015.06.013

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