A precaution when preparing very large plasmids by alkaline lysis procedure.

@article{Thomas1988APW,
  title={A precaution when preparing very large plasmids by alkaline lysis procedure.},
  author={Nadja Thomas and Sandeep Koshy and Mehmet Akif Şimşek and AbrahamK. Abraham},
  journal={Biotechnology and applied biochemistry},
  year={1988},
  volume={10 5},
  pages={
          402-7
        }
}
The extractabilities of plasmids of different sizes by the sodium lauryl sulfate (SDS)-alkali procedure were compared using either sodium acetate or potassium acetate buffer as the neutralizing agent. There was a selective loss of large plasmids (above 100 kb) when the potassium salt was used. When N-lauryl sarcosine instead of SDS was used as the detergent, no loss of large plasmids occurred in the presence of potassium salt. A comparison of the kinetics of precipitate formation with sodium… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-7 of 7 extracted citations