A pre-equilibrium before nucleotide binding limits fingers subdomain closure by Klentaq1.

@article{Rothwell2007APB,
  title={A pre-equilibrium before nucleotide binding limits fingers subdomain closure by Klentaq1.},
  author={Paul J. Rothwell and Gabriel Waksman},
  journal={The Journal of biological chemistry},
  year={2007},
  volume={282 39},
  pages={28884-92}
}
Numerous studies have been undertaken to establish the mechanism of dNTP binding and template-directed incorporation by DNA polymerases. It has been established by kinetic experiments that a rate-limiting step, crucial for dNTP selection, occurs before chemical bond formation. Crystallographic studies indicated that this step may be due to a large open-to-closed conformational transition affecting the fingers subdomain. In previous studies, we established a fluorescence resonance energy… CONTINUE READING

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