A practical in vitro sperm-egg binding assay that detects subfertile males.

@article{Barbato1998API,
  title={A practical in vitro sperm-egg binding assay that detects subfertile males.},
  author={Guy F. Barbato and P G Cramer and Roy H. Hammerstedt},
  journal={Biology of reproduction},
  year={1998},
  volume={58 3},
  pages={
          686-99
        }
}
A series of in vitro assays of sperm-egg binding were developed to identify potentially subfertile roosters. Initial assays used either segments of intact hen's egg perivitelline membrane (PVM) placed on a microscope slide or a heat-solubilized extract of PVM (HS-PVM) dried within a flat-bottomed microwell plate, with bound sperm detected with a DNA-specific stain and epifluorescence microscopy. An automated assay was developed using prestained sulfosuccinimidyl-7-amino-4-methylcoumarin-3… 
Identifying potentially subfertile toms via a sperm-binding assay.
TLDR
It is demonstrated that the sperm-egg binding assay detects differences in sperm quality between individuals, and these differences influence fertility.
Utilisation of sperm‐binding assay combined with computer‐assisted sperm analysis to evaluate frozen‐thawed bull semen
TLDR
Findings indicate that sperm‐egg‐binding assay associated with CASA is a reliable, practical and inexpensive method for examining the fertilising capacity of cryopreserved bull semen.
Sperm-binding to the perivitelline membrane of chicken egg yolk as a functional test for sperm evaluation in dogs
TLDR
The sperm-binding test to the perivitelline membrane of chicken egg yolk was considered a feasible sperm analysis test for both fertilizing capacity and overall sperm attributes evaluation, mainly when the analysis is performed by a conventional microscope, which expands its practicality to the majority of canine reproduction laboratories.
Increased in vitro binding and fertilizing ability of mouse sperm exposed to a synthetic peptide
TLDR
IVF rate was increased by exposure of mouse sperm to peptide at concentrations effective in the in vitro assay, and use of peptide allowed use of 1/10 as many sperm.
Exposure of human, boar, or bull sperm to a synthetic peptide increases binding to an egg-membrane substrate.
TLDR
Clearly, human, boar, and bull sperm were beneficially modified by brief in vitro exposure to FertPlus peptide, so that for many samples a greater percentage of sperm was bound in vitro.
Increased in vitro binding of fresh and frozen-thawed human sperm exposed to a synthetic peptide.
TLDR
For >30% of men, exposure of their sperm to this synthetic fragment of prosaposin at 1280 pM increased binding of sperm to an egg membrane substrate similar to that offered by the zona pellucida.
Towards a more precise assay of sperm function in egg binding.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 53 REFERENCES
Characterization and application of an avian in vitro spermatozoa-egg interaction assay using the inner perivitelline layer from laid chicken eggs.
TLDR
The perivitelline hydrolysis assay was able to detect more damaged spermatozoa in samples that had been either stored at 5 degrees C or cryopreserved in liquid N2 than did other tests of sperm quality, which are known to overestimate the fertilizing ability of stored avian semen.
Evaluation of semen from individual male domestic fowl by assessment of sperm: perivitelline interaction in vitro and in vivo.
1. Spermatozoa in semen samples from 8 individual male domestic fowls were shown to have a differential and characteristic ability to hydrolyse holes in the inner perivitelline layer from laid eggs
The effect of twenty-four hour in vitro storage on sperm hydrolysis through the perivitelline layer of ovipositioned turkey eggs.
TLDR
The number of sperm that hydrolyze through the perivitelline layer is reduced after in vitro storage of semen, showing higher fertility and hatchability of fertile eggs than for eggs that received the low dose but did not differ between fresh and stored treatments.
Determination of the capacity of ram epididymal and ejaculated sperm to undergo the acrosome reaction and penetrate ova.
TLDR
Comparisons of acrosomal integrity by electron or light microscopeopy were not different for sperm at any stage of epididymal maturation, suggesting that minimal nonspecific membrane changes occur and that light microscopy is valid for evaluating the acrosome status of ram spermatozoa.
Preferential attachment of cock spermatozoa to the perivitelline layer directly over the germinal disc of the hen's ovum.
TLDR
A correlation was demonstrated between sperm concentration and the number of spermatozoa attaching to the PL and undergoing an acrosome reaction and a simple quantitative technique was developed for assessing these events.
Homospermic versus heterospermic insemination of zona-free hamster eggs to assess fertility of fluorochrome-labeled acrosome-reacted bull spermatozoa.
TLDR
E egg penetration rates from heterospermic inseminations appear to be valuable indicators of fertility and much more sensitive predictors than results from homosper mic insemination studies.
Estimation of the relative fertilizing ability of frozen chicken spermatozoa using a heterospermic competition method.
TLDR
The heterospermic competition assay method is a powerful tool for estimating the relative fertilizing ability of the sperm cells in Rhode Island Red hens.
Binding of spermatozoa to the perivitelline layer in the presence of a protease inhibitor.
TLDR
It is demonstrated that sperm receptors exist in the PL over the germinal disc in the mature follicle in the quail ova.
CRC handbook of the laboratory diagnosis and treatment of infertility
TLDR
A critical Evaluation of Sperm Morphology Comparison with In Vitro Fertilization and Embryo Transfer Laboratory and Morphological Assessment of Human Oocytes and Early Concepti is presented.
...
1
2
3
4
5
...