A practical guide to quantify cell adhesion using single-cell force spectroscopy.

  title={A practical guide to quantify cell adhesion using single-cell force spectroscopy.},
  author={Jens Friedrichs and Kyle R. Legate and Rajib Schubert and Mitasha Bharadwaj and Carsten Werner and Daniel J M{\"u}ller and Martin Beno{\^i}t},
  volume={60 2},
Quantitative analysis of cellular interactions with the extracellular environment is necessary to gain an understanding of how cells regulate adhesion in the development and maintenance of multicellular organisms, and how changes in cell adhesion contribute to diseases. We provide a practical guide to quantify the adhesive strength of living animal cells to various substrates using atomic force microscopy (AFM)-based single-cell force spectroscopy (SCFS). We describe how to control cell state… CONTINUE READING

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