A practical guide to microfluidic perfusion culture of adherent mammalian cells.

@article{Kim2007APG,
  title={A practical guide to microfluidic perfusion culture of adherent mammalian cells.},
  author={Lily Nari Kim and Yi-Chin Toh and Joel Voldman and Hanry Yu},
  journal={Lab on a chip},
  year={2007},
  volume={7 6},
  pages={681-94}
}
Culturing cells at microscales allows control over microenvironmental cues, such as cell-cell and cell-matrix interactions; the potential to scale experiments; the use of small culture volumes; and the ability to integrate with microsystem technologies for on-chip experimentation. Microfluidic perfusion culture in particular allows controlled delivery and removal of soluble biochemical molecules in the extracellular microenvironment, and controlled application of mechanical forces exerted via… CONTINUE READING