A practical fluorogenic substrate for high-throughput screening of glutathione S-transferase inhibitors.

Abstract

We report a new fluorogenic substrate for glutathione S-transferase (GST), 3,4-DNADCF, enabling the assay with a low level of nonenzymatic background reaction. Inhibitors against Noppera-bo/GSTe14 from Drosophila melanogaster were identified by high throughput screening using 3,4-DNADCF, demonstrating the utility of this substrate.

DOI: 10.1039/c5cc02067k

Cite this paper

@article{Fujikawa2015APF, title={A practical fluorogenic substrate for high-throughput screening of glutathione S-transferase inhibitors.}, author={Yuuta Fujikawa and Fumika Morisaki and Asami Ogura and Kana Morohashi and Sora Enya and Ryusuke Niwa and Shinji Goto and Hirotatsu Kojima and Takayoshi Okabe and Tetsuo Nagano and Hideshi Inoue}, journal={Chemical communications}, year={2015}, volume={51 57}, pages={11459-62} }