A practical approach to generate suitable de novo synthesis RNA template for a flavivirus RNA-dependent RNA polymerase

Due to its high transcription efficiency, bacteriophage T7 RNA polymerase (T7 RNAP) has long been utilized in bacterial and in vitro systems to generate large quantity of RNA for various purposes (Studier and Moffatt, 1986). However, heterogeneity at the 3′-end of the RNA transcript remains a major limitation for in vitro RNA preparation using T7 RNAP. When… (More)