A practical approach to RT-qPCR-Publishing data that conform to the MIQE guidelines.

@article{Taylor2010APA,
  title={A practical approach to RT-qPCR-Publishing data that conform to the MIQE guidelines.},
  author={Sean J. Taylor and Michael Wakem and Greg Dijkman and Marwan Alsarraj and Marie Nguyen},
  journal={Methods},
  year={2010},
  volume={50 4},
  pages={S1-5}
}
Given the highly dynamic nature of mRNA transcription and the potential variables introduced in sample handling and in the downstream processing steps (Garson et al. (2009)), a standardized approach to each step of the RT-qPCR workflow is critical for reliable and reproducible results. The MIQE provides this approach with a checklist that contains 85 parameters to assure quality results that will meet the acceptance criteria of any journal (Bustin et al. (2009)). In this paper we demonstrate… CONTINUE READING
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