A powerful DNA extraction method and PCR for detection of microsporidia in clinical stool specimens.

@article{Mueller1999APD,
  title={A powerful DNA extraction method and PCR for detection of microsporidia in clinical stool specimens.},
  author={Andreas Mueller and K Stellermann and Pia Hartmann and Matthias Schrappe and Gerd F{\"a}tkenheuer and Bernd Salzberger and Volker Diehl and Caspar Franzen},
  journal={Clinical and diagnostic laboratory immunology},
  year={1999},
  volume={6 2},
  pages={243-6}
}
The diagnosis of intestinal microsporidiosis has traditionally depended on direct visualization of the parasite in stool specimens or intestinal biopsy samples by light and/or electron microscopy. Limited information about the specificity and sensitivity of PCR for the detection microsporidia in clinical stool specimens is available. To establish a sensitive and specific method for the detection of microsporidia in clinical samples, we studied clinical stool specimens of 104 randomly selected… CONTINUE READING
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