A potent anti-CD70 antibody-drug conjugate combining a dimeric pyrrolobenzodiazepine drug with site-specific conjugation technology.

@article{Jeffrey2013APA,
  title={A potent anti-CD70 antibody-drug conjugate combining a dimeric pyrrolobenzodiazepine drug with site-specific conjugation technology.},
  author={Scott C. Jeffrey and Patrick J. Burke and Robert P. Lyon and David W Meyer and Django Sussman and Martha E. Anderson and Joshua H. Hunter and Chris I Leiske and Jamie B. Miyamoto and Nicole D. Nicholas and Nicole M. Okeley and Russell J. Sanderson and Ivan J. Stone and Weiping Zeng and Stephen J Gregson and Luke Masterson and Arnaud C Tiberghien and Philip Howard and David E. Thurston and C L Law and Peter D. Senter},
  journal={Bioconjugate chemistry},
  year={2013},
  volume={24 7},
  pages={
          1256-63
        }
}
A highly cytotoxic DNA cross-linking pyrrolobenzodiazepine (PBD) dimer with a valine-alanine dipeptide linker was conjugated to the anti-CD70 h1F6 mAb either through endogenous interchain cysteines or, site-specifically, through engineered cysteines at position 239 of the heavy chains. The h1F6239C-PBD conjugation strategy proved to be superior to interchain cysteine conjugation, affording an antibody-drug conjugate (ADC) with high uniformity in drug-loading and low levels of aggregation. In… 
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