A post-embedding avidin—biotin peroxidase system to demonstrate the light and electron microscopic localization of lectin binding sites in rat kidney tubules

@article{Jones1986APA,
  title={A post-embedding avidin—biotin peroxidase system to demonstrate the light and electron microscopic localization of lectin binding sites in rat kidney tubules},
  author={Carolyn J. P. Jones and Robert William Stoddart},
  journal={The Histochemical Journal},
  year={1986},
  volume={18},
  pages={371-379}
}
A post-embedding method for the light and electron microscopic demonstration of lectin binding sites in rat kidney tubules is described. The use of biotinylated lectins, followed by treatment with avidin peroxidase and the DAB—H2O2 sequence, produced intense staining of acrylic sections at the electron microscope level: brush borders and associated structures, cytoplasmic granules, basal infoldings and basement membrane—plasmalemmal interfaces of proximal tubules bound… CONTINUE READING
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