A possible role of lipoxygenase in the activation of vanilloid receptors by anandamide in the guinea-pig bronchus.

@article{Craib2001APR,
  title={A possible role of lipoxygenase in the activation of vanilloid receptors by anandamide in the guinea-pig bronchus.},
  author={Susan J Craib and Heather C Ellington and Roger G Pertwee and Ruth A. Ross},
  journal={British journal of pharmacology},
  year={2001},
  volume={134 1},
  pages={30-7}
}
1. In the absence of indomethacin, anandamide did not contract the guinea-pig bronchus at concentrations up to 100 microM. In the presence of indomethacin (10 microM), anandamide induced concentration-related contractions with a pEC(50) value of 5.18+/-0.11. It was significantly less potent than capsaicin (pEC(50) 7.01+/-0.1). The anandamide uptake inhibitor AM404, produced only a 14.1+/-3.22% contraction at 100 microM. All experiments were conducted in the presence of PMSF (20 microM). 2. The… CONTINUE READING