A positive genetic selection for disrupting protein-protein interactions: identification of CREB mutations that prevent association with the coactivator CBP.

@article{Shih1996APG,
  title={A positive genetic selection for disrupting protein-protein interactions: identification of CREB mutations that prevent association with the coactivator CBP.},
  author={Hsueh Shih and Phyllis Goldman and Anthony J Demaggio and Stanley M. Hollenberg and Richard Goodman and Merl F. Hoekstra},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1996},
  volume={93 24},
  pages={13896-901}
}
The Escherichia coli tet-repressor (TetR) operator system was used to develop a variation of the yeast two-hybrid assay in which disruptions of protein-protein interactions can be identified by a positive selection. This assay, designated the "split-hybrid system," contains a two-component reporter. The first component contains LexA binding sites upstream of the TetR gene and the second contains TetR operator binding sites upstream of HIS3. Interaction of one protein fused to the LexA DNA… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 31 extracted citations

Similar Papers

Loading similar papers…