A position- and orientation-dependent element in the first intron is required for expression of the mouse hprt gene in embryonic stem cells.

@article{Magin1992APA,
  title={A position- and orientation-dependent element in the first intron is required for expression of the mouse hprt gene in embryonic stem cells.},
  author={Thomas Michael Magin and Carolanne McEwan and Margaret L. Milne and A M Pow and Jim Selfridge and David W. Melton},
  journal={Gene},
  year={1992},
  volume={122 2},
  pages={289-96}
}
The gene (hprt) coding for mouse HPRT (hypoxanthine phosphoribosyltransferase) is transcribed from a promoter lacking CAAT and TATAA boxes. It is expressed ubiquitously, albeit at different levels, in all tissues and cultured cells. During investigations to characterise hprt transcription control elements required in embryonic stem (ES) cells and to develop compact hprt minigenes for gene-targeting strategies, we discovered a requirement for intron-1 sequences for expression in ES cells. The… CONTINUE READING