A porcine reproductive and respiratory syndrome virus vaccine candidate based on PRRSV glycoprotein 5 and the Toll-like receptor 5 agonist Salmonella typhimurium flagellin.

@article{Song2015APR,
  title={A porcine reproductive and respiratory syndrome virus vaccine candidate based on PRRSV glycoprotein 5 and the Toll-like receptor 5 agonist Salmonella typhimurium flagellin.},
  author={Hongqin Song and Dan Xiong and Jing Wang and Xianyue Zhai and Guangcheng Liang},
  journal={Journal of molecular microbiology and biotechnology},
  year={2015},
  volume={25 1},
  pages={
          56-9
        }
}
Glycoprotein 5 (GP5) from porcine reproductive and respiratory syndrome virus (PRRSV) is a key inducer of neutralizing antibodies. A truncated GP5 gene lacking the signal peptide and transmembrane sequences was amplified via an overlap PCR method and inserted into prokaryotic expression vectors, pET32a or pGEX-6p-1, to add an His or GST tag, respectively. His-tagged GP5 was induced with IPTG, verified by SDS-PAGE and Western blotting, and purified to serve as an immunogen accompanied with the… CONTINUE READING
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