A polytopic membrane protein displays a reversible topology dependent on membrane lipid composition.

@article{Bogdanov2002APM,
  title={A polytopic membrane protein displays a reversible topology dependent on membrane lipid composition.},
  author={Mikhail V. Bogdanov and Phillip N Heacock and William Dowhan},
  journal={The EMBO journal},
  year={2002},
  volume={21 9},
  pages={2107-16}
}
To address the role of phospholipids in the topological organization of polytopic membrane proteins, the function and assembly of lactose permease (LacY) was studied in mutants of Escherichia coli lacking phosphatidylethanolamine (PE). PE is required for the proper conformation and active transport function of LacY. The N-terminal half of LacY assembled in PE-lacking cells adopts an inverted topology in which normally non-translocated domains are translocated and vice versa. Post-assembly… CONTINUE READING