A polished and reinforced thinned-skull window for long-term imaging of the mouse brain.

@article{Shih2012APA,
  title={A polished and reinforced thinned-skull window for long-term imaging of the mouse brain.},
  author={Andy Y. Shih and Celine Mateo and Patrick J. Drew and Philbert S. Tsai and David Kleinfeld},
  journal={Journal of visualized experiments : JoVE},
  year={2012},
  volume={61}
}
In vivo imaging of cortical function requires optical access to the brain without disruption of the intracranial environment. We present a method to form a polished and reinforced thinned skull (PoRTS) window in the mouse skull that spans several millimeters in diameter and is stable for months. The skull is thinned to 10 to 15 μm in thickness with a hand held drill to achieve optical clarity, and is then overlaid with cyanoacrylate glue and a cover glass to: 1) provide rigidity, 2) inhibit… CONTINUE READING
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