A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene

@article{Reddy1982APM,
  title={A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene},
  author={E. Premkumar Reddy and Roberta K. Reynolds and Eugenio Santos and Mariano Barbacid},
  journal={Nature},
  year={1982},
  volume={300},
  pages={149-152}
}
The genetic change that leads to the activation of the oncogene in T24 human bladder carcinoma cells is shown to be a single point mutation of guanosine into thymidine. This substitution results in the incorporation of valine instead of glycine as the twelfth amino acid residue of the T24 oncogene-encoded p21 protein. Thus, a single amino acid substitution appears to be sufficient to confer transforming properties on the gene product of the T24 human bladder carcinoma oncogene. 
Acquisition of transforming properties by alternative point mutations within c-bas/has human proto-oncogene
TLDR
The transforming gene of a human lung carcinoma-derived cell line, Hs242, has been cloned in biologically active form, and identified as c-bas/has, which results in the substitution of leucine for glutamine as amino acid 61 of the predicted protein. Expand
Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change
TLDR
The H-ras-1 gene cloned from T24 DNA induces transformation in NIH 3T3 cells, while the same gene cloning from normal cellular DNA does not, and the functionally significant difference appears to be a single base mutation. Expand
A point mutation in the last intron responsible for increased expression and transforming activity of the c-Ha-ras oncogene
The T24/EJ allele of the Ha-ras proto-oncogene owes its powerful oncogenic activity not merely to the well documented mutation that perturbs the structure of the encoded polypeptide, but in additionExpand
Mutation affecting the 12th amino acid of the c-Ha-ras oncogene product occurs infrequently in human cancer.
TLDR
The point mutation affecting the 12th amino acid of the c-Ha-ras gene product, while a valuable model for carcinogenesis, does not appear to play a role in the development of most human epithelial cancers of the bladder, colon, or lung. Expand
Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue
DNA sequence analysis of the activated oncogene from the T24 human bladder carcinoma line and two alleles of its normal cellular progenitor (c-Ha-ras-1) indicates that the genes encompass at leastExpand
Activation of Ki-ras2 gene in human colon and lung carcinomas by two different point mutations
Kirsten (Ki)-ras cDNA clones were prepared from human lung and colon carcinoma cell lines expressing an activated c-Ki-ras2 gene. DNA sequence analysis and transfection studies indicate thatExpand
Activation of Ha-ras p21 by substitution, deletion, and insertion mutations
The transforming activity of naturally arising ras oncogenes results from point mutations that affect residue 12 or 61 of the encoded 21-kilodalton protein (p21). By use of site-directed mutagenesis,Expand
Malignant activation of a K-ras oncogene in lung carcinoma but not in normal tissue of the same patient.
TLDR
The mutational event responsible for the malignant activation of a K-ras oncogene in a squamous cell lung carcinoma of a 66-year-old man was identified and appears to be specifically associated with the development of a human neoplasm. Expand
Nucleotide sequence analysis of the T24 human bladder carcinoma oncogene.
TLDR
The nucleotide sequence of the T24 human bladder carcinoma oncogene was determined, and the coding and noncoding sequences of the genome were identified, and it was shown that a single point mutation in the coding sequences ofThe T24 oncogen resulted in the acquisition of transforming properties. Expand
Homology between human bladder carcinoma oncogene product and mitochondrial ATP-synthase
TLDR
It is reported here that the amino acid sequence surrounding this residue is highly homologous to the β-subunit of mitochondrial and bacterial ATP-synthase in the region of the polypeptide that is believed to contribute to nucleotide binding. Expand
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References

SHOWING 1-10 OF 21 REFERENCES
Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells
TLDR
It is found that T24, a cell line derived from a human bladder carcinoma, can induce the morphological transformation of NIH 3T3 cells, and the gene responsible for this transformation is human in origin, <5 kilobase pairs in size and homologous to a 1,100-base polyadenylated RNA species found in T24 and HeLa cells. Expand
T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes
TLDR
A transforming gene isolated from T24 human bladder carcinoma cells is closely related to the BALB murine sarcoma virus (MSV) onc gene (v-bas), which implies that rather subtle genetic alterations have led to the activation of the normal human homologue of v-bas as a human transforming gene. Expand
Nucleotide sequence of the oncogene encoding the p21 transforming protein of Kirsten murine sarcoma virus.
TLDR
Nucleotide sequencing of a portion of this region has led to the identification of an open reading frame of 567 nucleotides coding for p21 kis protein, a virally encoded 21-kilodalton protein of Kirsten murine sarcoma virus. Expand
Human EJ bladder carcinoma oncogene is homologue of Harvey sarcoma virus ras gene
Examination of homologies between retroviral oncogenes and transforming sequences defined by transfection reveals that the human bladder carcinoma (EJ) oncogene is homologous to the Harvey sarcomaExpand
Transforming activity of human tumor DNAs.
  • T. Krontiris, G. Cooper
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1981
TLDR
It is suggested that dominant mutations or gene rearrangements can result in the activation of cellular transforming genes in some human tumors. Expand
Transforming genes of human bladder and lung carcinoma cell lines are homologous to the ras genes of Harvey and Kirsten sarcoma viruses.
TLDR
Results indicate that the transforming genes of these human tumor cell lines are the cellular homologs of two retroviral transforming genes. Expand
Tumorigenic transformation of mammalian cells induced by a normal human gene homologous to the oncogene of Harvey murine sarcoma virus
TLDR
Results indicate that high levels of a gene product encoded by a normal human oncogene can induce tumorigenic transformation. Expand
Cellular transforming genes.
TLDR
DNA's of various tumors induce transformation with high efficiencies, indicating that oncogenesis can involve dominant genetic alterations resulting in activation of cellular transforming genes. Expand
Isolation of a transforming sequence from a human bladder carcinoma cell line
TLDR
The oncogene appears to have undergone little, if any, amplification in several bladder carcinoma cell lines and is unrelated to transforming sequences detected in a variety of other types of human tumor cell lines derived from colonic and lung carcinoma and from neuroblastoma. Expand
Oncogenes in human tumor cell lines: molecular cloning of a transforming gene from human bladder carcinoma cells.
TLDR
The results indicate that a biologically active oncogene present in T24 human bladder carcinoma cells is molecularly cloned with lambda Charon 9A as the cloning vector. Expand
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