A point mutation in the transmembrane domain of the hemagglutinin of influenza virus stabilizes a hemifusion intermediate that can transit to fusion.

@article{Melikyan2000APM,
  title={A point mutation in the transmembrane domain of the hemagglutinin of influenza virus stabilizes a hemifusion intermediate that can transit to fusion.},
  author={Grigory B. Melikyan and Ruben M. Markosyan and Michael G. Roth and Fredric Cohen},
  journal={Molecular biology of the cell},
  year={2000},
  volume={11 11},
  pages={
          3765-75
        }
}
A hemagglutinin (HA) of influenza virus having a single semiconserved Gly residue within the transmembrane domain mutated to Leu (G520L) was expressed on cells; these cells were bound to red blood cells. By decreasing pH at 23 degrees C rather than 37 degrees C, an intermediate with properties expected of hemifusion just as the membranes are about to transit to full fusion was captured. As evidence: 1) increasing temperature to 37 degrees C at neutral pH allowed fusion to proceed; 2) after… CONTINUE READING

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