A platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine† †Electronic supplementary information (ESI) available: LC-MS, ES-MS and deconvoluted spectra for all reactions with proteins described herein, and 1H and 13C NMR spectra for all small molecule constructs. See DOI: 10.1039/c5ob01205h Click here for additional data file.

Abstract

All reagents were purchased from Sigma-Aldrich or Life Technologies and were used as received. Where described below pet. refers to petroleum ether (40–60 °C). All reactions were monitored by thin-layer chromatography (TLC) on pre-coated SIL G/UV254 silica gel plates (254 m) purchased from VWR. Flash column chromatography was carried out with Kiesegel 60 M… (More)

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@inproceedings{Smith2015APF, title={A platform for efficient, thiol-stable conjugation to albumin's native single accessible cysteine† †Electronic supplementary information (ESI) available: LC-MS, ES-MS and deconvoluted spectra for all reactions with proteins described herein, and 1H and 13C NMR spectra for all small molecule constructs. See DOI: 10.1039/c5ob01205h Click here for additional data file. }, author={Mark E. B. Smith and Mikael Bjerg Caspersen and Eifion Robinson and Maur{\'i}cio Morais and Antoine Maruani and Jo{\~a}o P. M. Nunes and K. M. Nicholls and Malcolm J. Saxton and Stephen Caddick and James R. Baker and Vijay Chudasama}, year={2015} }