A plasmid vector with positive selection and directional cloning based on a conditionally lethal gene.

@article{Yazynin1996APV,
  title={A plasmid vector with positive selection and directional cloning based on a conditionally lethal gene.},
  author={S. A. Yazynin and Sergey M. Deyev and M Jucovic and Robert W. Hartley},
  journal={Gene},
  year={1996},
  volume={169 1},
  pages={
          131-2
        }
}
A plasmid vector with a multiple cloning site (MCS) for positive selection of cloned inserts in Escherichia coli (Ec) has been devised, based on the expression plasmid (pMT416) for the bacterial ribonuclease barnase (Barn). The host is protected from the lethal effect of moderate expression of barn by expression of the gene bars, encoding its inhibitor, barstar (Bars), placed on the same plasmid. Full expression, however, is lethal. Induction is also lethal with the derived plasmid, pMT440… CONTINUE READING