A plasma membrane wound proteome: reversible externalization of intracellular proteins following reparable mechanical damage.

@article{Mellgren2010APM,
  title={A plasma membrane wound proteome: reversible externalization of intracellular proteins following reparable mechanical damage.},
  author={Ronald L. Mellgren},
  journal={The Journal of biological chemistry},
  year={2010},
  volume={285 47},
  pages={36597-607}
}
Cells in mechanically active tissues undergo constant plasma membrane damage that must be repaired to allow survival. To identify wound-associated proteins, a cell-impermeant, thiol-reactive biotinylation reagent was used to label and subsequently isolate intracellular proteins that become exposed on the surface of cultured cells after plasma membrane damage induced by scraping from substratum or crushing with glass beads. Scrape-damaged cells survived injury and were capable of forming viable… CONTINUE READING