A plant metallothionein produced in E. coli.

  title={A plant metallothionein produced in E. coli.},
  author={Peter Kille and Dennis R Winge and John L Harwood and John Kay},
  journal={FEBS letters},
  volume={295 1-3},
A metallothionein cDNA was generated from pea (Pisum sativum L.) roots, amplified by PCR and inserted into a plasmid for expression in E. coli. Purification of the resultant product generated 3 pools of cadmium-containing material after DEAE-cellulose chromatography. The amino acid composition of each was in excellent agreement with that predicted for pea metallothionein. A cadmium content of approximately 6 g.atoms per mole of protein was estimated. N-terminal sequence analysis revealed that… CONTINUE READING
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