A pilot study on post-thawing quality of Iberian red deer spermatozoa (epididymal and electroejaculated) depending on glycerol concentration and extender osmolality.

@article{MartnezPastor2006APS,
  title={A pilot study on post-thawing quality of Iberian red deer spermatozoa (epididymal and electroejaculated) depending on glycerol concentration and extender osmolality.},
  author={F Mart{\'i}nez-Pastor and F{\'e}lix Mart{\'i}nez and Vanesa Garc{\'i}a-Mac{\'i}as and Milagros Cristina Esteso and Enrique Anel and Mara R Fern{\'a}ndez-Santos and Ana Josefa Soler and P L P{\'e}rez de Paz and Juli{\'a}n Garde and Luis Anel},
  journal={Theriogenology},
  year={2006},
  volume={66 5},
  pages={1165-72}
}
The optimization of cryopreservation extenders is a fundamental issue for adequately performing germplasm banking on wild species. We have tested two glycerol concentrations (4 and 8%), and three extender osmolalities (320, 380 and 430 mOsm/kg; before adding cryoprotectants), for cryopreservation of epididymal and ejaculated sperm samples from Iberian red deer. All the extenders were based on Tes-Tris and fructose (for osmolality adjustment), and complemented with 20% egg yolk. Epididymal and… CONTINUE READING
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